Complex-Forming Properties of Spinach NADP+ Reductase with Ferredoxin, Ferrocyanide and NADP+
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منابع مشابه
Metabolic Interactions between Spinach Leaf Nitrite Reductase and Ferredoxin-NADP Reductase: Competition for Reduced Ferredoxin.
Steady state rates of NADP reduction decline upon commencement of nitrite reduction in reconstituted chloroplast preparations. Similarly, steady state rates of nitrite reduction are lower, but not zero, during concurrent NADP reduction. These results imply that competition for substrate occurs and suggest that nitrite reduction can successfully compete for reduced ferredoxin, even at high rates...
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1 7 W . K R E U T Z U. W . MENKE, Z . N a t u r f o r s c h . 1 7 b , 6 7 5 [1962], 18 W. KREUTZ, Z. Naturforsch. 18 b, 1098 [1963] ; 19 b, 441 [1964], 19 W. MENKE, in: Photosynthesis Mechanisms in green Plants, Publ. 1145, Nat. Acad. Sci.-Nat. Res. Council, Publ. 1963, p. 537. 20 W. MENKE, in: Biochemistry of Chloroplasts, Vol. I, (ed. T. GOODWIN), p. 3, Acad. Press, London and New York 1967. ...
متن کاملAlgal ferredoxin-NADP+ reductase with different molecular-weight forms.
Ferredoxin-NADP+ reductase from the microalga Bumilleriopsis was isolated by a combination of affinity chromatography on a flavodoxin-Sepharose 4 B column and usual purification procedures. Both the elution pattern of the final gel filtration step and of the sodium dodecylsulfate (SDS) disc gel electrophoresis indicate that there are at least two different molecular-weight forms of the reductas...
متن کاملFAD analogues as mechanistic and 'binding-domain' probes of spinach ferredoxin-NADP+ reductase.
The native flavin, FAD, of spinach ferredoxin--NADP+ reductase, has been replaced by a number of FAD analogues with modifications of the isoalloxazine ring system. The apoenzyme binds 8-mercapto-FAD in its thiolate anion form and 6-hydroxy-FAD in its neutral form. These results are consistent with classification of this enzyme as a dehydrogenase/electron transferase, an ascription originally ma...
متن کاملProbing the function of the invariant glutamyl residue 312 in spinach ferredoxin-NADP+ reductase.
Ferredoxin-NADP+ reductase, the prototype of a large family of structurally related flavoenzymes, pairs single electrons carried by ferredoxin I and transfers them as a hydride to NADP+. Four mutants of the enzyme, in which Glu-312 was replaced with Asp, Gln, Leu, and Ala to probe the role of the residue charge, size, and polarity in the enzyme activity, have been heterologously expressed, puri...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1980
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1980.tb04695.x